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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Cytometry A. 2010 Aug;77(8):776–782. doi: 10.1002/cyto.a.20925

Figure 3.

Figure 3

Fluorescence images of HL4-MG and EGFP fusions to transmembrane proteins. Left column shows representations of fusion protein topology in the plasma membrane, where top is extracellular and bottom is intracellular. (A-C) NIH 3T3 cells expressing human β2AR with FAP or EGFP fused to the N-terminus. (D-F) C2C12 cells expressing human GLUT4 with extracellular FAP or EGFP, plus new transmembrane domain, fused to the N-terminus. (G-I) HEK 293 cells expressing CFTR with FAP or EGFP, plus new transmembrane domain, fused to the N-terminus. . (A, D, G) Cells are imaged in the presence of impermeable fluorogen, MG-11P. (B, E, H) Membrane permeant fluorogen, MG-ester, labels intracellular fusion proteins. Scale bars represent 10μm.