Table 1.
Primary and secondary antibodies used in this study
| Target | Origin | Company/Source | Catalog no. | Dilution |
|---|---|---|---|---|
| S phase cells (BrdU) |
Rat monoclonal | Abcam | ab6326 BU1/75(ICR1) |
2 µg/mL |
| Rat IgG (H+L)1 | Rabbit polyclonal | Vector Laboratories |
BA-4000 | 1 µg/mL |
| Proliferating cell nuclear antigen (PCNA) |
mouse monoclonal | Dako | M0879, clone PC10 | 1:1,000 |
| CuZnSOD (SOD1) | rabbit polyclonal | Lab Frontier | LF-PA0013 | 1:10,000 |
| MnSOD (SOD2) | rabbit polyclonal | Stressgen | SOD-110 | 1:5,000 |
| EC-SOD (SOD3) | rabbit polyclonal | custom-made3 | ref # [80] | 1 µg/mL |
| Peroxiredoxin 1 (PRDX1) |
rabbit polyclonal | Lab Frontier | LF-PA0001 | 1:2,000 |
| Peroxiredoxin 3 (PRDX3) |
rabbit polyclonal | Lab Frontier | LF-PA0030 | 1:2,000 |
| Thioredoxin 1 (TXN1) |
rabbit polyclonal | Lab Frontier | LF-PA0002 | 1:2,000 |
| Thioredoxin 2 (TXN2) |
rabbit polyclonal | Lab Frontier | LF-PA0012 | 1:1,000 |
| Glutathione peroxidase 1 (GPX1) |
rabbit polyclonal | Lab Frontier | LF-PA0019 | 1:1,000 |
| Catalase (CAT) | mouse monoclonal | Sigma | C0979, clone CAT- 505 |
1:40,000 |
| Cytosolic aconitase (ACO1) |
rabbit polyclonal | R. Eisenstein | ref # [81] | 1:2,000 |
| Mitochondrial aconitase (ACO2) |
rabbit polyclonal | R. Eisenstein | ref # [82] | 1:2,000 |
| IDH24 | rabbit polyclonal | custom-made3 | 0.5 µg/mL | |
| NNT5 | rabbit polyclonal | custom-made3 | 1 µg/mL | |
| Nitrotyrosine | mouse monoclonal | Upstate | 05–233, clone 1A6 | 1:2,000 |
| β-actin2 | mouse monoclonal | Sigma | A3854, clone AC-15 | 1:50,000 |
| IRS-1 | rabbit polyclonal | Cell Signaling | 2382 | 1:1,000 |
| Phospho-IRS-1 (Ser332/336) |
rabbit polyclonal | Cell Signaling | 2580 | 1:1,000 |
| β-Arrestin 1 | mouse monoclonal | Invitrogen/Zymed | 39–5000 | 1:1,000 |
| Phospho-β-Arrestin 1 (Ser412) |
rabbit polyclonal | BioSource | 44–200 | 1:1,000 |
| Casein kinase 1ε | mouse monoclonal | Santa Cruz | Sc−81446 | 1:1,000 |
| ERK1/2 | rabbit polyclonal | Assay Designs | KAP-MA001 | 1:1,000 |
| Phospho-ERK1/2 (Thr202/Tyr204) |
rabbit polyclonal | Assay Designs | KAP-MA021 | 1:1,000 |
| PI3K P110α | rabbit polyclonal | Cell Signaling | 4255 | 1:1,000 |
| Rb | rabbit polyclonal | Abcam | Ab6075 | 1:200 |
| Phospho-Rb (Ser807) |
rabbit polyclonal | Invitrogen | 44–579 | 1:1,000 |
| P34 Cdc2 | rabbit polyclonal | Invitrogen/Zymed | 33–1800 | 1:1,000 |
| Phospho-p34 Cdc2 (Thr14/Tyr15) |
rabbit polyclonal | Invitrogen/Zymed | 38–7300 | 1:1000 |
| Mouse IgG (H+L)2 | goat polyclonal | Bio-Rad | 172–1011 | 1:10,000 |
| Rabbit IgG (H+L)2 | goat polyclonal | Bio-Rad | 170–6516 | 1:10,000 |
1
tagged with biotin
2
tagged with HRP
3
Proteintech Group Inc., USA
4
Keyhole limpet hemocyanin conjugated (KLH)-N433TTDFLDTIKSNLDRALGKQ452 peptide as antigen
5
KLH-C1073DALQAKVRESYQK1086 peptide as antigen