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. 2010 Sep 24;5(9):e12971. doi: 10.1371/journal.pone.0012971

Figure 4. MALDI-TOF/MS spectra of peptides generated from mouse hnRNP A3 in complex with hnFIX-AIE RNA and mFIX-AIE RNA, and with no AIE RNA.

Figure 4

A. Cropped mass spectra of the 2DE protein spot containing the UV-irradiated and RNase-treated 32P-hFIX-AIE RNA/hnRNP A3 complex. Mass-spectrum m/z 1910.8 corresponding to a tryptic peptide of hnRNP A3 (aa 356 through 377) with Ser359 unphosphorylated was reproducibly detected, while mass-spectrum m/z 1990.8 corresponding to the same peptide with phosphorylated Ser359 was not detected. B. Cropped mass spectra of the 2DE protein spot containing the UV-irradiated and RNase-treated 32P-mFIX-AIE RNA/hnRNP A3 complex. Experimental conditions used and observations made were similar to those described in A. C. Cropped mass spectra of one of protein spots separated in 2DE of liver NEs (free of hnRNP-AIE RNA). Both spectra m/z 1910.9 and m/z 1990.8 were reproducibly detected.