Table 2. Effects of siRNA 6 on hFIX expression from HepG2 cells transfected with -416FIXm1 (containing no AIE) and -416FIXm1/1.4 (containing AIE).

		Relative expression Activity (% ± SD)*
siRNA	MOI	-416FIIX m1	-416FIIX m1/1.4
PBS-saline		100	100
Scrambled siRNA 6	2	105±16	100±17
siRNA 6	2	109±17	96±9
Scrambled siRNA 6	20	100±15	98±6
siRNA 6	20	109±21	86±23
Scrambled siRNA 6	100	108±23	98±16
siRNA 6	100	104±25	64±21#

*HepG2 cells transfected with hFIX minigene expression vector, -416FIXm1 (containing no AIE) or -416FIXm1/1.4 (containing AIE), were treated with PBS-saline, or transduced with adenoviral particles harboring scrambled siRNA 6 or siRNA 6, and hFIX protein produced was measured (n = 4). Human FIX expression activity was normalized to that of HepG2 cells treated with PBS-Saline for each expression vector condition. SD, standard deviation. #P<0.05 (vs. scrambled siRNA6).