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A: RM samples of 2 µl were analyzed by 16% SDS-PAGE and immunoblotted using anti-His antibodies. B: Solubility of D-CF expressed mAQP4 M23 in presence of 0.2% Brij-35, 0.4% Digitonin, 0.1% Triton X-100, and 0.05% Tyloxapol. Control is P-CF expressed mAQP4 M23. S, supernatant; P, pellet.
