Table 1.
List of different libraries and culture conditions together with library statistics
| Library | Short name | Strains | Condition/mediuma | cDNAs | Contigs | Singletons | TUs | %Rb |
|---|---|---|---|---|---|---|---|---|
| Original standardc | OS | Pt1 clone 8.6 (CCAP1055/1) | 12,136 | 3,274 | 1,165 | 4,439 | 67.31 | |
| Silica plus | SP | Pt1 clone 8.6 (CCAP1055/1) | 350 uM metasilicate in ASW | 7,508 | 3,077 | 384 | 3,461 | 57.21 |
| Silica minus | SM | Pt1 clone 8.6 (CCAP1055/1) | No metasilicate addition | 6,968 | 2,838 | 459 | 3,297 | 54.63 |
| Oval morphotype | OM | Pt3 (CCAP1052/1B) | Low salinity (10% ASW) | 4,544 | 2,202 | 214 | 2,416 | 48.78 |
| Nitrate replete | NR | Pt1 clone 8.6 (CCAP1055/1) | 1.12 mM in chemostat | 3,632 | 2,028 | 242 | 2,270 | 39.01 |
| Nitrate starved | NS | Pt1 clone 8.6 (CCAP1055/1) | 50 μM for 3 days in chemostat | 9,122 | 3,271 | 512 | 3,783 | 60.79 |
| Ammonium adapted | AA | Pt1 clone 8.6 (CCAP1055/1) | 75 μM | 9,031 | 3,329 | 567 | 3,896 | 60.20 |
| Urea adapted | UA | Pt1 clone 8.6 (CCAP1055/1) | 50 μM | 8,552 | 3,157 | 464 | 3,621 | 59.82 |
| Tropical accession | TA | Pt9 (CCMP633) | Grown at 15°C | 4,821 | 2,015 | 160 | 2,175 | 56.95 |
| Low decadienal | LD | Pt1clone 8.6 (CCAP1055/1) | 0.5 μg/m 2E,4E-decadienal for 6 h | 9,227 | 3,322 | 537 | 3,859 | 61.65 |
| High decadienal | HD | Pt1 clone 8.6 (CCAP1055/1) | 5 μg/m 2E,4E-decadienal for 6 h | 3,541 | 1,734 | 323 | 2,057 | 44.95 |
| Iron limited | FL | Pt1 clone 8.6 (CCAP1055/1) | 5 nM | 8,264 | 3,064 | 487 | 3,551 | 59.19 |
| Triradiate morphotype | TM | Pt8 (CCAP1055) | 12,566 | 3,055 | 520 | 3,575 | 70.49 | |
| Blue light | BL | Pt1 clone 8.6 (CCAP1055/1) | 48 h dark adapted cells exposed to 1 h blue light | 12,045 | 4,253 | 607 | 4,860 | 59.61 |
| CO2 high 4 days | C4 | Pt1 clone 8.6 (CCAP1055/1) | 3.2 mM DIC for 4 days in chemostat | 10,283 | 3,564 | 160 | 3,724 | 63.78 |
| CO2 high 1 day | C1 | Pt1 clone 8.6 (CCAP1055/1) | 3.2 mM DIC for 1 day in chemostat | 10,307 | 3,598 | 165 | 3,763 | 63.49 |
aAll cells grown in artificial seawater media, except chemostat cultures, which were grown in Walne medium [54]. bPercent redundancy of sequences in each library. cThe original P. tricornutum cDNA library described previously [21,22] is herein referred to as OS. Although incorporated into the comparative expression analyses, it was not examined extensively because it was generated using a different cDNA library protocol. ASW (artificial sea water),; TU, transcriptional unit.