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Single-tube nested PCR products with primer pairs TFR-3/TFR-4 and TFITS-F/TFITS-R confirming the presence of Tritrichomonas foetus organism in rectal swab samples of the 2 female and male littermate Siamese cats by amplification of the 208-bp product. M, molecular weight marker; R, female Siamese cat; B, male Siamese littermate cat; C, culture medium.
