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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Mol Cell Neurosci. 2010 Jul 24;45(3):258–266. doi: 10.1016/j.mcn.2010.06.017

Fig 1.

Fig 1

Early exposure to mShh-N and 10−8M retinoic acid promotes midbrain regionalization of human ES cell-derived neural progenitor cells. (A) Human ES cells were differentiated by 4 concentrations of RA with mouse Shh for 12 days before exposure to BDNF, ascorbic acid, mouse Shh and Fgf8b for 8 days. (B) Quantitative PCR determined that 10−8M retinoic acid with mouse Shh induced a midbrain-like transcriptional profile by significantly increasing the levels of EN1 without changing the levels of OTX2, HOXB1 and PAX2 (* p < 0.05 ANOVA).

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