Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Time course for the hydrolysis of racemic 5 using two mutants of PTE. The total concentration of compound 5 was determined by the complete hydrolysis using 0.1 M KOH. The hydrolysis of the SP-enantiomer of racemic 5 was initiated with 165 nM H254G/H257W/L303T of PTE. After 10 minutes, the G60A mutant of PTE was added to hydrolyze the RP-enantiomer.
