Skip to main content
. Author manuscript; available in PMC: 2011 Sep 21.
Published in final edited form as: Biochemistry. 2010 Sep 21;49(37):7978–7987. doi: 10.1021/bi101056m

Figure 3.

Figure 3

Hydrolysis of 250 μM racemic GB followed by ITC. All reactions were initiated by injection of 5 μL of GB into a volume of 200 μL and followed with a MicroCal iTC200 isothermal calorimeter. (A) Hydrolysis of racemic GB by 30 nM wild-type PTE as a function of time. The data are fit to single exponential. (B) Hydrolysis of GB by 60 nM G60A as function of time. The data are fit to the sum of two exponentials. (C) Hydrolysis of racemic GB by 10 nM H257Y/L303T mutant as function of time. The data are fit to the sum of two exponentials.