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. Author manuscript; available in PMC: 2011 Sep 21.
Published in final edited form as: Biochemistry. 2010 Sep 21;49(37):7978–7987. doi: 10.1021/bi101056m

Table 1.

Turnover numbers (kcat, s−1) for the wild type and mutant forms of PTE.

Compound WT G60A I106G F132G S308G H254Q/
H257F		 H257Y/
L303T		 I106G/
H257Y		 I106G/
F132G/
H257Y		 I106A/
F132A/
H257Y		 I106A/
H257Y/
S308A		 H254G/
H257W/
L303T
Rp/Sp-1 3.9e2 1.6e2 1.8e2 1.8e2 - 3.8e1 1.7e2 4.8e2 1.5e2 7.9e2 2.3e3 2.7e2
Rp-1 1.5e2 1.3e2 6.8e1 6.8e1 1.1e2 1.7e2 7.3e0 8.9e1 3.4e1 N/D 2.0e2 1.4e1
Sp-1 6.7e2 1.1e2 3.0e2 3.0e2 2.9e2 3.2e1 4.1e2 6.8e2 3.5e2 9.0e2 1.4e3 1.9e2
Rp/Sp-2 9.7e1 1.1e2 3.9e1 2.9e1 1.8e2 1.2e1 3.2e2 5.9e1 5.3e1 1.6e2 6.5e2 1.2e2
Rp-2 1.0e2 1.2e2 4.0e1 3.9e1 2.5e2 4.8e1 1.8e1 4.7e1 - 4.5e1 1.3e2 -
Sp-2 4.0e1 7.4e0 2.8e1 1.6e1 1.5e1 7.2e0 3.7e2 1.0e2 4.8e1 5.1e2 3.4e2 9.2e1
Rp/Sp-3 5.4e1 4.7e1 4.9e1 3.5e1 1.2e2 1.1e1 1.3e2 4.7e2 - 6.3e2 6.7e2 4.7e1
Rp-3 9.3e1 3.7e1 3.1e1 2.8e1 1.1e2 7.0e1 5.1e1 8.4e0 - - 2.2e1 2.0e1
Sp-3 2.2e1 - 4.8e1 2.5e1 1.2e1 6.3e0 1.0e2 2.1e2 2.3e2 2.3e2 5.3e2 5.0e1
RpRc/RpSc
/SpRc/SpSc-4 		2.1e0 4.1e0 1.2e0 - 1.1e0 1.3e0 2.4e0 2.4e-1 2.9e-2 4.3e-1 1.1e0 1.8e1
RpRc/SpRc-4 2.5e0 3.9e0 - - 2.5e0 8.2e-1 3.0e0 4.8e-1 4.2e-2 5.0e-1 7.8e-1 1.1e1
RpSc/SpSc-4 6.1e-1 1.3e0 9.0e-2 1.3e-1 4.2e-1 4.2e-1 8.0e-1 1.6e-2 4.1e-2 1.1e-1 1.4e-1 4.6e0
RpRc-4 3.4e0 8.5e0 3.3e0 1.2e1 2.2 e0 5.5e-1 4.1e-1 7.6e-2 4.9e-2 5.0e-2 1.7e-1 2.0e0
RpSc-4 4.5e-1 1.5e0 1.5e-1 1.5e-1 6.2e-1 1.7e-1 - 6.5e-2 5.5e-3 - 4.2e-2 2.1e-1
SpRc-4 7.7e-1 9.5e2 5.0e-2 3.2e-1 5.0e-1 6.3e-1 6.3e0 3.7e-2 1.6e-1 7.4e-1 1.0e0 1.2e1
SpSc-4 1.6e-2 - - 1.2e-1 1.2e-1 3.3e-1 2.1e0 2.2e-2 5.3e-2 2.4e-1 3.5e-1 2.9e0
Rp/Sp-5 2.8e1 1.9e1 1.1e1 5.4e0 8.4e1 3.8e1 4.5e0 7.8e0 1.6e1 2.4e0 - 1.3e1
Rp-5 - 9.3e1 - 1.4e1 1.5e2 3.8e1 5.9e0 - - - 4.2e0 8.1e-1
Sp-5 - - 5.1e0 3.3e-1 1.4e-1 - 5.1e0 1.8e1 1.3e1 4.7e0 4.5e0 1.9e1