Table 3.
Ratios of kcat/Km for the hydrolysis of chiral substrates by the wild-type and mutant forms of PTE.
| Compound | (Rp:Sp) | (Rp:Sp) | (Rp:Sp) | (RpRc:RpSc:SpRc:SpSc) | (Rp:Sp) |
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | |
| wild-type | 1:2 | 22:1 | 25:1 | 400:63:35:1 | 760:1 |
| G60A | 4:1 | 84:1 | 7600:1 | 3600:700:20:1 | 23000:1 |
| I106G | 1:1 | 18:1 | 1:1 | 38:5:1.5:1 | 6:1 |
| F132G | 1:9 | 11:1 | 3:1 | 64:8:16:1 | 35:1 |
| S308G | 1:2 | 34:1 | 19:1 | 340:73:17:1 | 280:1 |
| H254Q/H257F | 1:5 | 1:2 | 1:5 | 3:1:29:1 | 16:1 |
| H257Y/L303T | 1:64 | 1:44 | 1:6 | 4:1:120:16 | 1:3 |
| I106G/H257Y | 1:12 | 1:3 | 1:64 | 8:1:3:1 | 1:7 |
| I106G/F132G/H257Y | 1:18 | 1:5 | 1:200 | 7:1:11:4 | 1:89 |
| I106A/F132A/H257Y | 1:51 | 1:22 | 1:270 | 19:1:120:19 | 1:14 |
| I106A/H257Y/S308A | 1:12 | 1:4 | 1:41 | 13:1:54:7 | 2:1 |
| H254G/ H257W/ L303T | 1:140 | 1:33 | 1:120 | 2:1:65:14 | 1:110 |