Table 1.
Genes upregulated in the control CF intestine as compared to control WT, and downregulated by lubiprostone treatment of CF mice.
| CF | WT+L | CF+L | Symbol | Name | Function/Process |
|---|---|---|---|---|---|
| 1176 | 1.52 | 365 | Retnlb | resistin like beta | Innate immunity |
| 112 | 3.11 | 4.5 | Chi3l4 | chitinase 3-like 4 | Innate immunity |
| 51 | 0.52 | 8.7 | Pla2g4c | phospholipase A2, group IVC (cytosolic, calcium- independent) | Inflammation, eicosanoid metabolism |
| 42 | 0.76 | 3.4 | Mcpt1 | mast cell protease 1 | Innate immunity, mast cell |
| 23 | 1.32 | 2.8 | Spna1 | spectrin alpha 1 | Erythrocyte cytoskeleton |
| 18 | 0.23 | 1.3 | Gsdmc2 | gasdermin C2 | Growth, differentiation, apoptosis |
| 16 | 0.76 | 1.4 | Mcpt2 | mast cell protease 2 | Innate immunity, mast cell marker |
| 14 | 1.15 | 2.1 | Mcpt9 | mast cell protease 9 | Innate immunity, mast cell |
| 12 | 1.32 | 3.7 | B3galt5 | UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 | Sialyl Lewis-a synthesis |
| 11 | 4.92 | 2.1 | Ube2l6 | ubiquitin-conjugating enzyme E2L 6 | Innate immunity, ISG15- ubiquitination |
| 8.6 | 1.00 | 1.2 | Capn13 | calpain 13 | Growth, differentiation, apoptosis |
| 8.0 | 0.23 | 1.4 | Gsdmc4 | gasdermin C4 | Growth, differentiation, apoptosis |
| 7.0 | 0.27 | 1.3 | Gsdmc | gasdermin C | Growth, differentiation, apoptosis |
| 4.9 | 0.44 | 1.4 | Cpa3 | carboxypeptidase A3, mast cell | Innate immunity, mast cell marker |
| 4.0 | 0.76 | 0.2 | Fcer1a | Fc receptor, IgE, high affinity I, alpha polypeptide | Innate immunity, mast cell marker |
| 3.7 | 1.15 | 1.4 | Egln3 | EGL nine homolog 3 (C. elegans) | Prolyl hydroxylation of HIF |
| 3.5 | 1.62 | 1 | (none) | single chain antibody ScFv | Immunoglobulin heavy chain, immunity |
| 3.2 | 0.56 | 0.5 | Scd2 | stearoyl-Coenzyme A desaturase 2 | Unsaturated fatty acid synthesis |
Genes whose expression level in the CF control intestine were ≥ 3-fold greater than WT control and were downregulated ≥ 3-fold by lubiprostone in CF mice. Expression values are data from the GCOS analysis relative to WT control levels. When there were multiple probe sets for a particular gene the values were averaged. Gene names are according to the Mouse Genome Informatics website http://www.informatics.jax.org/. +L = lubiprostone treated. Equal amounts of total RNA were pooled from 7-10 mice in each group and were used to interrogate the Affymetrix Mouse 430 2.0 GeneChip (n = 1 per group). The full microarray dataset is deposited in NCBI's Gene Expression Omnibus and is accessible through GEO Series accession number GSE18327 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18327.