Table 2.
Genes downregulated in the control CF intestine as compared to control WT, and upregulated by lubiprostone treatment of CF mice.
| CF | WT+L | CF+L | Symbol | Name | Function/Process |
|---|---|---|---|---|---|
| 0.01 | 0.4 | 0.11 | Slc5a12 | solute carrier family 5 (sodium/glucose cotransporter), member 12 | Na-monocarboxylate transporter |
| 0.05 | 0.4 | 0.33 | Cyp4a10 | cytochrome P450, family 4, subfamily a, polypeptide 10 | 20-HETE synthesis |
| 0.08 | 0.9 | 0.46 | Pck1 | phosphoenolpyruvate carboxykinase 1, cytosolic | Glucose metabolism |
| 0.12 | 0.6 | 1.1 | Pdk4 | pyruvate dehydrogenase kinase, isoenzyme 4 | Glucose metabolism |
| 0.15 | 1.3 | 0.53 | Fbp1 | fructose bisphosphatase 1 | Glucose metabolism |
| 0.21 | 1.2 | 2.14 | Hmgcs2 | 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 | Ketogenesis |
| 0.21 | 1.1 | 1.74 | Acot2 | acyl-CoA thioesterase 2 | Fatty acid metabolism |
| 0.25 | 1.1 | 0.93 | Slc13a2 | solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2 | Na-dicarboxylate transporter |
| 0.25 | 1.1 | 1.33 | Sgk1 | serum/glucocorticoid regulated kinase 1 | Regulation of electrolyte transport |
| 0.33 | 1.6 | 1.32 | Akr1b7 | aldo-keto reductase family 1, member B7 | PGF2a synthesis |
| 0.33 | 1.1 | 2.15 | Angptl4 | angiopoietin-like 4 | Lipid metabolism |
Genes whose expression level in the CF control intestine were ≥ 3-fold less than WT control and were upregulated ≥ 3-fold by lubiprostone in CF mice. Expression values are data from the GCOS analysis relative to WT control levels. Gene names are according to the Mouse Genome Informatics website http://www.informatics.jax.org/. +L = lubiprostone treated. Equal amounts of total RNA were pooled from 7-10 mice in each group and were used to interrogate the Affymetrix Mouse 430 2.0 GeneChip (n = 1 per group). The full microarray dataset is deposited in NCBI's Gene Expression Omnibus and is accessible through GEO Series accession number GSE18327 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE18327.