Table 2.
Top 10 gene expression alterations by Libby six-mix (15×106 μm2/cm2) in LP9/TERT-1 and HKNM-2 mesothelial cells
| Gene Name (Abbreviation) | Fold Changea | LP9/TERT-1 qRT-PCR Validation |
HKNM-2 qRT-PCR Resultsb | |
|---|---|---|---|---|
| Increased: | 8 h | 24 h | ||
| Tissue factor pathway inhibitor-2 (TFPI2) | NC | 11* | -- | -- |
| Interleukin 8 C-terminal variant, 211506_s_t (IL8) | NC | 9* | -- | 3 |
| Prostaglandin-endoperoxide synthase 2 (PTGS2) | NC | 7* | -- | -- |
| Interleukin 8 (IL8) | NC | 7* | 14* | -- |
| Pyruvate dehydrogenase kinase, isozyme 4 (PDK4) | NC | 7* | -- | -- |
| Pleckstrin homology-like domain, family A, member 1 (PHLDA1) | NC | 5* | -- | -- |
| Chemokine (C-X-C motif) ligand 3 (CXCL3) | NC | 5 | -- | -- |
| Activating transcription factor 3 (ATF3) | NC | 5* | 5* | 5 |
| Superoxide dismutase 2, mitochondrial (SOD2) | 4* | 5* | -- | 2 |
| Annexin 14 (ANX14) | NC | 4 | -- | -- |
| -- | ||||
| Decreased: | -- | |||
| Oxytocin receptor (OXTR) | NC | 5* | -- | -- |
| Chromosome 5 open reading frame 13 (C5orf13) | NC | 4* | -- | -- |
| Chromosome 21 open reading frame 7 (C21orf7) | NC | 3* | -- | -- |
| Cytochrome P450, family 24, subfamily A, polypeptide 1 (CYP24A1) | NC | 3* | -- | -- |
| Calponin 1, basic, smooth muscle (CNN1) | NC | 3 | -- | -- |
| Cyclin-dependent kinase inhibitor 1C (CDKN1C) | NC | 3 | -- | -- |
| Methyltransferase like 7A (METTL7A) | NC | 2* | -- | -- |
| Periplakin (PPL) | NC | 2* | -- | -- |
| Kelch-like 4 (KLHL4) | NC | 2 | -- | -- |
| Phospholipase C-like 1 (PLCL1) | NC | 2* | -- | -- |
a NC = No significant (p < 0.05) change greater than 2-fold from untreated controls across all 3 replicates.
bHKNM-2 normal human pleural mesothelial cells were treated with 15×106 μm2/cm2 Libby asbestos for 24 h.
*Gene in the top 10 genes upregulated/downregulated by crocidolite asbestos at the same time and concentration [16].