Fig. 5.

VHHs ALc-B8 and ALc-D4 co-localize with BoNT/A protease when co-expressed within cultured neuronal cells. All images are of M17 neuroblastoma cells transfected with one or two expression plasmids as indicated. After four days post-transfection the cells were viewed by fluorescence microscopy for YFP-VHH (green channel) or CFP-BoNT/A Lc (red channel) to determine the distribution of the fusion proteins. An overlay is shown that represents areas of green and red color co-localization as orange. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)