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. Author manuscript; available in PMC: 2011 Mar 23.
Published in final edited form as: Nature. 2010 Sep 23;467(7314):484–488. doi: 10.1038/nature09395

Figure 5.

Figure 5

Stopped-flow transport assay of reconstituted CusA with extravesicular Ag+ ion. (a) Mutants of the methionine-residue relay network. The decrease in fluorescence signal of PGSK mediated by proteoliposomes of wild-type CusA indicates the transport of Ag+ across the membrane. The stopped-flow traces are the cumulative average of four successive recordings (wild-type CusA, black curve; M486I, green curve; M391I, brown curve; M573I, red curve; M623I, purple curve; M672I, orange curve; M755I, cyan curve; control liposome, blue curve). (b) Mutants of the proton-relay network. The traces are the cumulative average of four successive recordings (D405A, blue curve; E939A, red curve; K984A, green curve).