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. 2010 Aug 4;151(10):4756–4764. doi: 10.1210/en.2010-0505

Figure 3.

Figure 3

Opioid ligand and receptor gene expression. mRNA expression levels of the opioid prepropeptides (left) was examined in the PFC, NAc, and hypothalamus (HYP) of offspring from HF-fed dams (black bars) and control mice (gray bars) using qRT-PCR. PENK (NAc, PFC, and hypothalamus) and POMC (hypothalamus) were significantly up-regulated in mice from dams fed the HF diet. mRNA expression levels of opioid receptor genes OPRD1 (δ), OPRK1 (κ), and OPRM1 (μ) in the NAc, PFC, and hypothalamus of offspring from HF-fed dams (black bars) and control mice (gray bars) was determined by qRT-PCR (right). Mice from HF-fed dams demonstrate a significant up-regulation of μ-opioid receptor. Expression level of each gene examined was normalized to the GAPDH and β-actin levels and expressed relative to the control group set to 1. Values are mean ± sem. *, P values <0.05 (n = 6 per group).