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. 2010 Aug 25;151(10):4994–5006. doi: 10.1210/en.2010-0428

Figure 3.

Figure 3

qPCR analysis of genes related to ovulation and luteinization in WT (white bars) and Inha−/− (black bars) preovulatory granulosa cells collected from mice treated with and without hCG. Preovulatory granulosa cells were collected 44–46 h after PMSG injection without (−) or with hCG for 6 h. qPCR results are expressed as RQ. A, Lhcgr is suppressed in Inha−/− preovulatory granulosa cells with and without hCG. B, Pgr is up-regulated similarly between WT and Inha−/− preovulatory granulosa cells treated with hCG. C, Adamts1, regulated at ovulation by LH and progesterone receptor (56), is not up-regulated in Inha−/− cells. Data are mean ± sem of a minimum of three independent samples with the control mean set to 1. Different letters above the bars indicate statistical significance at P < 0.05 by one-way ANOVA and followed by Fisher LSD post hoc tests.