Table 1.

Proton Chemical Shifts (ppm) for the Z–F Duplex^a

base	H8/H6	H5/Me/H2	H1′	H2′	H2″	H3′	H4′	H1/H3	NHb/NHnbb
C1	7.65	5.90	5.77	1.99	2.43	4.72	4.38		c
G2	7.98		5.93	2.70	2.77	5.00	4.38	13.07
C3	7.40	5.46	5.64	2.06	2.42	4.88	4.38		6.56/8.38
A4	8.36	7.64	6.26	2.70	2.90	5.05	4.45
T5	7.17	1.53	5.84d	1.72	2.22	4.81d	4.42	13.35
F6	7.04	1.76	4.84	2.01	2.22	4.78	4.38	5.86
G7	7.83		5.91	2.65	2.76	4.95	4.38	12.25
T8	7.27	1.29	6.02	2.13	2.54	4.85	4.38	13.82
T9	7.40	1.66	5.73	2.12	2.47	4.90e	4.46	13.71
A10	8.34	7.47	6.21	2.75	2.87	5.05	4.45
C11	7.37	5.37	5.94	2.42	2.08	4.70	4.45		6.75/8.22
C12	7.62	5.66	6.21	2.26	2.26	4.54	4.31		c
G13	7.89		5.71	2.58	2.71	4.83	4.41	c
G14	7.85		6.02	2.65	2.80	4.99	4.41	12.84
T15	7.27	1.46	5.62	2.07	2.39	4.90e	4.42	13.52
A16	8.26	6.99	5.94	2.75	2.89	5.08	4.42
A17	8.11	7.53	6.03	2.54	2.78	5.03	4.43
C18	7.17	5.27	5.45	1.51	1.89	4.68	4.42		6.89/8.00
Z19	8.14	1.83/6.09	5.87	2.61	2.72	4.98	4.44	6.59/5.84
A20	8.11	7.58	6.11	2.62	2.86	4.99	4.44
T21	7.08	1.30	5.69	1.99	2.37	4.85	4.38	13.45
G22	7.88		5.86	2.62	2.70	4.98	4.38	12.70
C23	7.36	5.42	5.79	1.93	2.36	4.83	4.38		6.67/8.44
G24	7.96		6.17	2.64	2.39	4.69	4.43	c

^aNon-exchangeable proton chemical shifts were measured at 15 °C and referenced to the HDO signal at 4.90 ppm, the exchangeable proton chemical shifts were measured at 3 °C and referenced to the HDO signal at 5.05 ppm.

^bThe abbreviations NH_b and NH_nb refer to the hydrogen-bonded and non-hydrogen-bonded amino protons in cytosine.

^cChemical shift not determined due to exchange broadening.

^dAssignment made 40 °C.

^eAssignment made at 20 °C.