TABLE 1.
Protein composition of the human pre-mRNA 3′ processing comple
| Protein Name | Accession # | Yeast homolog |
Motifs/notes on functions |
Cal. Mass |
# of peptides |
|
|---|---|---|---|---|---|---|
| L3 | SVL | |||||
| KNOWN POLYADENYLATION FACTORS | ||||||
| CPSF Complex | ||||||
| CPSF160 (CPSF1) | NP_037423 | CFT1 | SFT1 | 160822 | 80 | 72 |
| CPSF100 (CPSF2) | NP_059133.1 | CFT2 | β-CASP | 88487 | 59 | 52 |
| CPSF73 (CPSF3) | NP_057291 | YSH1 | β-CASP | 77486 | 42 | 22 |
| CPSF30 (CPSF4) | NP_006684 | YTH1 | Zinc finger | 30124 | 18 | 13 |
| hFip1 | NP_112179 | FIP1 | 66526 | 23 | 19 | |
| CstF Complex | ||||||
| CstF77 (CSTF3) | NP_001317 | RNA14 | HAT | 82922 | 60 | 54 |
| CstF64 (CSTF2) | NP_001316 | RNA15 | RRM | 60959 | 31 | 22 |
| CstF50 (CSTF1) | NP_001315 | WD repeats | 48358 | 21 | 18 | |
| CF Im Complex | ||||||
| CF Im 68 (CPSF6) | NP_008938 | RRM | 59209 | 15 | 10 | |
| CF Im 59 | NP_079087 | RRM | 52050 | 15 | 12 | |
| Cf Im 25 (CPSF5) | NP_008937 | 26227 | 26 | 21 | ||
| CF IIm Complex | ||||||
| Pcf11 | NP_056969.2 | PCF11 | CID | 173050 | 5 | 3 |
| Other known polyadenylation factors | ||||||
| Symplekin | NP_004810 | PTA1 | 126500 | 51 | 39 | |
| Symplekin variant | BAE06092.1 | 118793 | 48 | 38 | ||
| PAPOLG | NP_075045 | PAP | RRM | 82803 | 2 | 3 |
| PABPC1 | NP_002559.2 | PAB1 | RRM | 70671 | 27 | 33 |
| PABPC4 | NP_003810.1 | RRM | 0783 | 20 | 23 | |
| PABPN1 | NP_004634 | RRM | 31749 | 14 | 12 | |
| (PUTATIVE) HOMOLOGUES OF YEAST POLYADENYLATION FACTORS | ||||||
| CstF64 tau (CSTF2T) | NP_056050.1 | SPAC644.16 (S. pombe) |
RRM | 64436 | 21 | 16 |
| WDR33 | NP_060853 | PFS2 | WD repeats | 145921 | 49 | 45 |
| RBBP6 | NP_008841 | MPE1 | RS, DWNN | 201563 | 4 | 3 |
| PP1 alpha | NP_002699.1 | GLC7 | Phosphatase | 37512 | 2 | 7 |
| PP1 beta | NP_002700 | GLC7 | Phosphatase | 37187 | 2 | 5 |
| DNA DAMAGE RESPONSE FACTORS | ||||||
| DNA-PK | NP_008835 | Kinase | 469093 | 21 | 15 | |
| Ku 70 | NP_001460 | YKU70 | DNA helicase | 69843 | 4 | 7 |
| Ku 86/XRCC5 | NP_066964 | YKU86 | DNA helicase | 82705 | 6 | 13 |
| PARP-1 | NP_001609 | 113135 | 5 | |||
| RNAP II AND ASSOCIATED FACTORS | ||||||
| Pol II large subunit Rpb1 |
NP_000928.1 | RPB1 | Polymerase | 217204 | 2 | 2 |
| Pol II B | NP_000929.1 | RPB2 | 133896 | 2 | ||
| Rpb5 (Pol II E) | NP_002686.2 | RPB5 | 24611 | 2 | ||
| Rpb11 | AF468111_1 | RPB11 | 17208 | 2 | ||
| Transcription Factors | ||||||
| TF II, I | NP_127492.1 | 112416 | 8 | 2 | ||
| TAF15 | AAH46099.1 | RRM, zf-RanBP | 52061 | 6 | 5 | |
| Integrator Complex | ||||||
| INTS2 | NP_065799.1 | 134346 | 2 | 2 | ||
| INTS3 | NP_075391.3 | 118013 | 4 | 9 | ||
| INTS4 | NP_291025.3 | 108171 | 2 | 3 | ||
| INTS6 | NP_036273.1 | 100390 | 2 | 3 | ||
| INTS7 | NP_056249.1 | 106834 | 2 | 2 | ||
| INTS8 | NP_060334.2 | 113088 | 3 | |||
| INTS9 | NP_060720.1 | YSH1 homology | 73815 | 4 | ||
| INTS10 | NP_060612.2 | 82236 | 2 | |||
| PAF Complex | ||||||
| Cdc73 | AAH14351.2 | CDC73 | 46675 | 2 | 2 | |
| FACT Complex | ||||||
| FACT complex large subunit |
NP_009123 | SPT16 | 119914 | 4 | 4 | |
| SPLICING FACTORS | ||||||
| p54nrb | NP_031389.3 | 54232 | 11 | 3 | ||
| PSF | NP_005057.1 | 76150 | 10 | 4 | ||
| PUF60 | NP_510965.1 | RRMs | 59876 | 3 | 9 | |
| UAP56 | NP_004631.1 | SUB2 | RNA Helicase | 48991 | 12 | 9 |
| SF1 | NP_004621.2 | BBP | 68330 | 5 | 5 | |
| SF3A, subunit 1 | NP_005868.1 | PRP21 | Surp | 88886 | 9 | 15 |
| SF3A, subunit 2 | NP_009096.2 | PRP11 | 49256 | 2 | 4 | |
| SF3A60 | CAA57388 | PRP9 | 58777 | 3 | 18 | |
| SF3B, subunit 1 | NP_036565.2 | HSH155 | 145830 | 16 | 34 | |
| SF3B, subunit 2 | NP_006833.2 | CUS1 | 100228 | 2 | 21 | |
| SF3B, subunit 4 | NP_005841.1 | HSH49 | 44386 | 3 | 5 | |
| SF3B, 14 kDa | NP_057131.1 | SNU17 | 14585 | 5 | ||
| Prpf38b | NP_060531.1 | 64441 | 2 | 8 | ||
| SRm300 | NP_057417.2 | RS | 299676 | 13 | 15 | |
| U1 70K | NP_003080.2 | SNP1 | RRM | 51557 | 7 | 7 |
| U2AF65 | NP_009210.1 | MUD2 | RRM | 53501 | 6 | 11 |
| U2AF35 | NP_006749.1 | RRM | 27872 | 2 | 3 | |
| PRP19 | NP_055317.1 | PRP19 | RING, WD | 55181 | 5 | 3 |
| EXOSOME | ||||||
| SKIV2L2 (hMTR4) | NP_056175.2 | MTR4 | DEXD | 117933 | 29 | 28 |
| TRANSLATION FACTORS | ||||||
| eEF1-alpha | NP_001393.1 | TEF1/2 | GTPase | 50141 | 14 | 12 |
| eEF1-gamma | NP_001395.1 | TEF4 | GST | 50119 | 5 | 4 |
| eIF2A | NP_114414.2 | YGR054W | 64990 | 4 | 4 | |
| eIF 4A | NP_001407.1 | TIF1 | Helicase | 46154 | 12 | 6 |
| eIF3 gamma | NP_003747.1 | JAB/MPN | 39930 | 5 | 6 | |
| eIF3S2 | NP_003748.1 | TIF34 | WD | 36502 | 2 | 2 |
| eIF3S5 | NP_003745.1 | PCI | 37564 | 3 | 2 | |
| eIF3S6 | NP_001559.1 | 52221 | 2 | 4 | ||
| eIF3S9 eta | NP_003742.2 | RRM | 92482 | 7 | 7 | |
| eIF3A | NP_003741.1 | RPG1 | 166569 | 16 | 9 | |
| eIF4G1 | NP_886553.2 | TIF4631 | 175460 | 2 | 2 | |
| RACK1/GNB2L1/lung cancer oncogene 7 |
NP_006089.1 | ASC1 | WD | 35077 | 5 | 5 |
| FACTORS WITH KNOWN MOTIFS | ||||||
| RNA binding motif protein 7 |
NP_057174.1 | RRM | 30503 | 4 | 4 | |
| RNA binding motif protein 9/Ataxin- binding protein/Fox-1 |
NP_055124 | RRM | 39515 | 2 | 7 | |
| RNA binding motif protein 25 |
NP_067062.1 | RRM | 100186 | 4 | 16 | |
| RNA binding motif protein 39, isoform a |
NP_909122.1 | RRM | 59380 | 2 | 4 | |
| RNA binding motif protein 39, isoform b |
NP_004893.1 | RRM | 58657 | 2 | 4 | |
| DEAH box polypeptide 3 |
NP_001347.3 | DBP1 | RNA helicase | 7244 | 13 | 9 |
| DEAH box | NP_004387.1 | DBP2 | RNA helicase | 69148 | 4 | 5 |
| polypeptide 5 | ||||||
| DEAH box polypeptide 9 |
NP_001348.2 | RNA helicase | 140958 | 32 | 3 | |
| DEAH box polypeptide 15 |
NP_001349.2 | PRP43 | RNA helicase | 90933 | 3 | 17 |
| DEAH box polypeptide 36 |
NP_065916.1 | RNA helicase | 114776 | 12 | 27 | |
| DEAH box polypeptide 39 |
NP_005795.2 | RNA helicase | 49130 | 8 | 5 | |
| DEAD box polypeptide 6 |
NP_004388.1 | DHH1 | RNA helicase | 82432 | 3 | 2 |
| DEAD box polypeptide 17 (p82) |
NP_006377.2 | RNA helicase | 80273 | 6 | 4 | |
| Gemin3 (DEAD box polypeptide 20) |
NP_009135.3 | RNA helicase | 92213 | 4 | 2 | |
| DEAD box polypeptide 23 |
NP_004809.2 | PRP28 (S. Pombe) |
RNA helicase | 95583 | 4 | 5 |
| ZCCHC8 | NP_060082 | Zinc finger | 79375 | 15 | 16 | |
| EBNA2 coactivator, p100/SND1 |
NP_055205.2 | Staphylococcal nuclease domain, Tudor |
101997 | 12 | 2 | |
| Interferon-induced protein with tetratricopeptide repeats 1 isform 2 |
NP_001539.3 | TPR | 55360 | 9 | 7 | |
| Interferon-induced protein with tetratricopeptide repeats 3 |
NP_001540 | TPR | 55985 | 8 | 5 | |
| OTHER FACTORS | ||||||
| G3BP/Ras-GTPase- activating protein SH3-domain-binding protein |
NP_005745.1 | Endoribonuclease | 52164 | 5 | 2 | |
| JUP protein/ gamma- catenin |
NP_002221.1 | 81745 | 5 | 3 | ||
| PNUTS (PPP1R10) | NP_002705.2 | PP1 regulator | 99058 | 6 | 9 | |
*
Components of multi-subunit complexes that are present in only one purified complexe are listed and lightly shaded.
*
Proteins that are abundant in H complexes were designated H complex components and not listed here.