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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Mol Cell. 2010 Aug 13;39(3):444–454. doi: 10.1016/j.molcel.2010.07.032

Figure 1. Kin4 Inhibits the MEN in every Cell Cycle.

Figure 1

(A) Wild-type (A2587), lte1Δ (A24807), bub2Δ (A23045), kin4Δ (A17865), lte1Δ bub2Δ (A24808) and lte1Δ kin4Δ (A24806) cells were spotted on YePAD plates and incubated at 30°C and 16°C. Pictures shown represent growth from 2 days for the 30°C condition and 10 days for 16°C. The first spot represents growth of approximately 3×104 cells and each subsequent spot is a 10 fold serial dilution.

(B) Cells in (A) were arrested in G1 with 5 µg/mL α factor and released into pheromone free media at room temperature. After 70 minutes, 10 µg/mL α factor was added to prevent entry into the subsequent cell cycle. Cells were collected every 15 minutes and stained for tubulin by indirect immuno-fluorescence and for the DNA with DAPI. Anaphase was determined by spindle and nuclear morphology. n ≥ 100 cells.