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. Author manuscript; available in PMC: 2010 Sep 27.
Published in final edited form as: J Cell Physiol. 2010 Sep;224(3):827–836. doi: 10.1002/jcp.22191

Fig.4. 4HPR-induced neuronal differentiation is blocked by C/EBPβ overexpression.

Fig.4

ARPE-19 cells were treated with 1µM 4HPR for indicated time, and the total RNA preparations were analyzed by real-time quantitative PCR as described under Materials and Methods. The values are mean ± SD, n = 4. *P < 0.001 compared with control. **P < 0.001 compared with 4HPR treatment. Panel a, the time-dependent change in C/EBPβ mRNA expression during 4HPR-induced neuronal differentiation of RPE cells. Panel b, C/EBPβ overexpression blocked 4HPR-induced neuronal differentiation. Cells in culture were transfected with C/EBPβ expression vector, and after 24 h post-transfection the cells were treated with 1 µM 4HPR for 72 h, and then analyzed by phase contrast microscopy. Scale bar = 100 µm. Panel c, C/EBPβ transfection blocked calretinin mRNA expression induced by 4HPR. Cells were transfected with C/EBPβ expression vector and treated as described in panel b. Panel d, C/EBPβ expression inhibits 4HPR-induced decrease of IGFBP5 mRNA expression. Cells were transfected with C/EBPβ expression vector and treated as described in panel b.