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. Author manuscript; available in PMC: 2010 Sep 27.
Published in final edited form as: Nutr Cancer. 2009;61(5):746–753. doi: 10.1080/01635580902825597

Figure 3. Stearate induces cleavage and activation of caspase-3 in the Hs578t cells.

Figure 3

A) Beginning approximately 6 hours after stearate treatment, cleaved caspase-3 appeared and was present 24 hours post-treatment. Concurrent with cleaved caspase-3 was the presence of cleaved PARP, a well-characterized downstream target of caspase-3. The immunoblots are representative of 5 independent experiments. B) To confirm that stearate is activating caspase-3 in a time dependent manner, caspase-3 activity was measured 6, 12 and 24 hours after treatment. Stearate significantly increased caspase-3 activity approximately 12 hours post-treatment. (n=3-4; **p<0.03, *p<0.05 by ANOVA). C) Stearate induces caspase-3 activity in dose dependent manner (n=3-4; *p<0.04, **p<0.005 by ANOVA).