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. Author manuscript; available in PMC: 2010 Sep 27.
Published in final edited form as: Clin Exp Metastasis. 2009 Mar 8;26(5):415–424. doi: 10.1007/s10585-009-9239-x

Figure 6. Stearate Induced Apoptosis of MDA-MB-435 Breast Cancer Cells In Vitro.

Figure 6

A) Cells were treated with 50 μM stearate for the times indicated and the presence of cleaved caspase-3 and cleaved PARP was determined by immunblot. As shown, stearate induces cleavage of the two proteins 12-24 hours post-treatment indicating stearate is inducing apoptosis of the breast cancer cells (n=3). B) Cells were treated with 1-100 μM stearate for 12 hours and caspase-3 activity was measured using a fluorescence based assay. Stearate activated caspase-3 in a dose dependent manner (n=4; *p<0.023 ANOVA).