Figure 4.

Survival curves of mice re-challenged i.p. with 5 × 10⁵ viable B16F1 tumor cells. Pre-vaccinated mice surviving the first tumor challenge were without any additional treatment re-challenged 100 days after the first tumor challenge. Control: mock treated; 1 × 90: vaccine + two additional injections of CpG ODNs applied once 90 days before tumor inoculation; 2 × 90: vaccine + two additional injections of CpG ODNs applied twice 90 and 75 days before tumor inoculation; 3 × 90: vaccine + two additional injections of CpG ODNs applied three times 90, 75 and 60 days before tumor inoculation; 1 × 60: vaccine + two additional injections of CpG ODNs applied once 60 days before tumor inoculation; 2 × 60: vaccine + two additional injections of CpG ODNs applied twice 60 and 45 days before tumor inoculation; 3 × 60: vaccine + two additional injections of CpG ODNs applied three times 60, 45 and 30 days before tumor inoculation; 1 × 30: vaccine + two additional injections of CpG ODNs applied once 30 days before tumor inoculation; 2 × 30: vaccine + two additional injections of CpG ODNs applied twice 30 and 15 days before tumor inoculation; 3 × 30: vaccine + two additional injections of CpG ODNs applied three times 30, 15 and 7 days before tumor inoculation. The experiment was repeated three times.