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Effects of 27-OHC on TNF-α levels and NFκB. Treatment with 27-OHC increases TNF-α levels, as shown by ELISA (a) and increased NFκB levels in the nucleus as shown by Western blot (b). Immunocytochemistry for NFκB shows increased immunoreactivity (green) and translocation into the nucleus (DAPI, blue) of this protein in the 27-OHC-treated cells (arrows, c). *p < 0.05 vs control; Bar, 20 μm.
