Figure 7.

Effect of simvastatin on the serum-free induced cell viability of bone marrow -derived MSCs in vitro. Cells were incubated with vehicle or simvastatin plus wortmannin (W), a PI3-K inhibitor, or simvastatin alone for 24 h prior to viability assessment. Wortmannin was added 0.5 h prior to simvastatin. A. Histographic representation of nuclear DNA contents measured by flow cytometry. AP = Apoptotic cells. B. The percentage of apoptotic MSCs analyzed by flow cytometry. C. Cell viability analyzed by MTT. Means ± SD. n = 6 wells per group. The data are representative of 3 individual experiments. * p < 0.05 versus control group. # p < 0.05 versus simvastatin + W group.