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. 2010 Sep 17;9:249. doi: 10.1186/1476-4598-9-249

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Copyright ©2010 Abdulamir et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Examples of ISH staining of mRNA of IL-1, IFN-γ, COX-2, IL-8, c-Myc, and Bcl-2. Cells stained positive are shown with dark color of NBT/BCIP in the nuclei of glandular cells as well as stromal cells. mRNA expression of IL-1 (A-C) at X100, IFN-γ (D-F) at 400X, COX-2 (G-I) at X100, and IL-8 (J-L) at 100X, is shown in control, TU SGMB+ve-CRCw/bac, and TU SGMB-ve-CRC-w/bac, respectively. mRNA expression of c-Myc (M-O) at X400 and Bcl-2 (P-R) at X400 is shown in control, TU SGMB-ve-CRC-wo/bac, and NTU SGMB-ve-CRC-wo/bac.