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. 2010 Sep 21;9:253. doi: 10.1186/1476-4598-9-253

Figure 2.

Figure 2

Expression of mTrop2 leads to increased migration, foci formation and anchorage-independent growth. (a) Panc02-mTrop2 and control cells were serum starved for 24 h followed by the addition of media containing either 0% or 5% FBS. Wounds were generated in confluent cell monolayers utilizing a sterile pipette tip. Pictures were taken 24 h after the wound insult. A representative field for each cell line is shown. (b) The expression of mTrop2 led to a loss of contact inhibition and foci formation. Foci greater than 1 mm were counted after 16 days. Mean ± SD is shown (n = 3). ** P < 0.0001. (c) Expression of mTrop2 on the more indolent murine pancreatic adenocarcinoma cell line, Panc03, also led to increased foci formation (arrows). (d) Panc02-mTrop2 cells acquire an enhanced ability to grow in soft agar. Panc02-mTrop2 and control cells were plated in 6-well plates as described in the Methods section. Colonies were measured after 72 h to determine the increased rate of colony formation in Panc02-mTrop2 cells. Results are shown as mean ± SD (n = 3). *P < 0.001.