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Impact of metabolic impairment on the expression of nanE and siaP. 2019ΔcyaA and derived double mutants were grown in sRPMI. Sialic acid and cAMP were added 30 min prior to RNA extraction. Expression of nanE and siaP were measured by qRT-PCR. Results are presented as fold change relative to a culture that received neither sialic acid nor cAMP.
