Table 2.

Oligonucleotide primers

Oligonucleotide primer	Sequence1	Use
140F3	gaa ttc CTG CTT CTT CAT TAA GTT CTC GC	nagA upstream
140R5	ccc ggg CAT ATT CTG TTC CTA ATA TCA ACA TCA GTT	nagA upstream
140F6	ccc ggg TAA TAG TAA ACA CTT AAA TAG TTA ATT GAT TTA AAA ATC	nagA downstream
140R6	gca tgc TCA AAA ACA GCA ACA CGG TGC	nagA downstream
141F1	gaa ttc CAT CAT CGC TGA AAC AGG C	nagB upstream
141R3	ccc ggg CAT ATT AGC CTT CCT TTA TTA TTG ACC G	nagB upstream
141F3	ccc ggg TAG AGA TCT ATT CTT CAT CTT TAT GTA GGG	nagB downstream
141R4	gca tgc GGT TTC AAC GCT AGT TTG GTC G	nagB downstream
144F1	gaa ttc CCG TCC TTT TGT GAA TGT CC	nanK upstream
144F3	ccc ggg CAT AAC TTA TCC TTA TAG TGT AAA GTC TTT TCT CAC	nanK upstream
144F2	ccc ggg AAG CCT GAA GGA ACA ATT TAT GGC TAA	nanK downstream
144R2	gca tgc GCC GTT TCA GCA GAA TAA CCA G	nanK downstream
145F5	gaa ttc CGC TCC TGT GTG AAC TTA TG	nanE upstream
145R5	ccc ggg CAT ATA ACA CCC CTC ATT TAA ATC TGA AT	nanE upstream
145F6	ccc ggg TAG TCG TAA GAC GTG TGA GAA AAG ACT T	nanE downstream
145R6	gca tgc CGA ACG CAA AAT CGT ATC GGC	nanE downstream
604F1	gag ctc CAT TTT GCT GAC GAG GAA CTG	cyaA upstream
604R1	ccc ggg CAT TAC ATA AAC ACC TAA AAT TGG TGG	cyaA upstream
604F2	ccc ggg TAA TAT TTT CCT GTG GTT GAT AGG TTA CC	cyaA downstream
604R2	aag ctt AAA GCA ATG GAG TGG ACC ACA ATT	cyaA downstream
145R8	CCG CAG CAA TTT TTG TCC	PCR SOEing
145M2	TTT ATG AAA AAA CAC TTC AAA AAT	PCR SOEing
145M3	ATT TTT GAA GTG TTT TTT CAT AAA TTA AAT GTG ATC AAC TTC TC	PCR SOEing
146R2	CCA TTA CGG CAC ACT AAA GAG G	PCR SOEing
957F2	aag ctt AAT AAA ACG GAA TTT TTG AAA CAG G	CRP expression
957R2	ctc gag TCT TGC GCC ATA TAC AAC GAT TGT	CRP expression
P146F1	ACA CCC CTC ATT TAA ATC TGA ATA AAT CAC	EMSA
P146R4	CCC CCA AAA TAG GAT TCG	EMSA
145R7	CGA CAG GTT GGC AAG AAG AAA TAA GAC C	Primer extension
145R1	ATC AGC GGC AAG AAC AGC AG	Primer extension

¹Restriction sites added to aid in subcloning are indicated by lowercase letters. Bases added for 5 bp insertion are italicized.