Figure 3.

Over-expression of THBS4 significantly decreases colony formation in CRC cell lines. Forced over-expression of THBS4 significantly decreases colony formation in colorectal cancer cell lines regardless of their baseline expression levels. The average reduction in colony formation following THBS4 over-expression was 50-60% compared with vector-only controls. This consistent and significant reduction in colony forming ability may indicate that high levels of this protein could correlate with reduced tumour cell growth.