Figure 5. Septin deletion mutants are more sensitive to BFA.

A. Spot test of tenfold serial dilutions of wild type and septin deletion mutants grown at 22°C onto YPD with brefeldin A (BFA, 25 µM) or its solvent, methanol. Septin mutants were more sensitive than wild type cells to BFA. B. Wild type and septin deletion mutants cells were grown to log phase at 22°C in YPD and treated with BFA (200 µM) or its solvent methanol for 12 h. BFA treatment mildly affected wild type cell morphology. In contrast, septin mutant cells became rounded and lysated. In both cases a cell separation delay was observed. Bar: 10 µm.