Table 2. Localization of Sep2-GFP fusion in different septin mutant backgrounds.
Localization | Number of cells (%) | |||
wt | sep1Δ | sep3Δ | sep4Δ | |
Bud neck | 70.4±1.4 | 2.8±0.7 | 4.9±0.1 | 72.7±1.0 |
Bud tip | 41.6±1.7 | 38.1±0.6 | 2.4±0.8 | 33.7±0.2 |
nuclear | 0 | 0 | 93.3±1.7 | 0 |
mislocalized1 | 0 | 21.2±1.8 | 0.7±0.9 | 0 |
Non-specific2 | 29.6±1.9 | 40.7±2.2 | 1.1±1.2 | 27.3±2.0 |
A total of more than 100 cells per experiment (3 independent experiments) were counted per each mutant phenotype (p = 0.001).
Accumulation of signal outside of bud neck, bud tip or nuclear localization. For instance in cell walls.
Diffuse signal in cytoplasm.