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. 2010 Sep 27;5(9):e13040. doi: 10.1371/journal.pone.0013040

Figure 2. Effects of USP7 NTD and CTD on p53 DNA binding.

Figure 2

(A) Schematic representation of the USP7 proteins used in this study showing the N-terminal (NTD) or TRAF domain, central catalytic (CAT) and C-terminal (CTD) domains. The position of the C223S point mutation that inactivates the catalytic domain is also shown. (B–C) EMSAs showing titration of latent p5382–393, in the presence or absence of 20 µM USP7-NTD (B) or 20 µM USP7-CTD (C). Quantification of the discreet shifted bands are shown in the graphs, with USP7 in black and BSA in grey.