Figure 2. Histological analysis of the islets in IRE1α CKO mice and control mice.

(A) The upper, middle, and lower panels show HE-stained sections, immunostained images using anti-insulin antibodies, and immunostained images using anti-glucagon antibodies, respectively. The three images for the control or IRE1α CKO mice are derived from the serial sections (scale bar: 50 µm). (B) ^aThe number of islets counted per 1 cm² of pancreas section is shown as mean ± S.E.M (n = 10 pancreas sections). ^bThe diameter (μm) of islet is shown as mean ± S.E.M (n = 50). ^cThe ratio of insulin⁺ cells to glucagons⁺ cells per islet is shown as mean ± S.E.M (n = 10). All the data were obtained from 20 weeks old female mice.