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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Neurosurg. 2010 Sep;113(3):656–665. doi: 10.3171/2010.3.JNS091627

Figure 7. Detection of 5-HT in the dorsal raphe nucleus slice.

Figure 7

A. Photograph of the slice chamber, WINCS unit with rechargeable battery, stimulating electrode and CFM. B. Cartoon drawing showing the position of the CFM and stimulating electrode in the brain slice (DR: dorsal raphe, Aq: Aqueduct). C. Pseudo-color plot for electrically evoked 5-HT (40 pulses). Black bar indicates time of stimulation (HFS: High-frequency stimulation). D. Electrically evoked 5-HT levels elicited by trains of 10, 20 and 40 pulses. INSET: Background-subtracted voltammogram for a 40-pulse train. E. Electrically evoked 5-HT levels before and after the application of 5 μM fluoxetine (20 pulses). Stimulation pulses (200 μA, 2 ms duration) were applied at a 60 Hz frequency.