Figure 6. Ethacrynic acid, H₂O₂, serum or glucose deprivation mediated neuronal injuries were attenuated by sirtinol or 2-hydroxynaphthaldehyde, and these injuries were potentiated by resveratrol or fisetin.

Near-pure neuronal cultures were exposed to A) 25 µM ethacrynic acid (ETH), or 80 µM H₂O₂ for 24 hrs, or B) to 5 hrs of glucose deprivation, or continuous serum deprivation (36 hrs) and neuronal death was assessed by LDH release to the bathing medium (mean ± SEM, n = 6–18 cultures per condition). Fisetin and resveratrol exposures were a 3 hr pretreatment at 10 µM. Sirtinol and 2-hydroxynaphthaldehyde were present chronically as indicated at 10 µM. * indicates difference from 25 µM ETH; # indicates difference from 80 µM H₂O₂; $ indicates difference from 5 hours of glucose deprivation exposure; and & indicates difference from serum deprivation exposure at P < 0.05 by one-way ANOVA followed by a Bonferroni test.