Table 1. NAD⁺ levels are reduced by SD and oxidant exposures, and are restored by pyruvate, nicotinamide, and TPEN.

Near-pure cortical neuronal cultures were exposed and total cellular NAD⁺ was isolated at the times indicated which are prior to neuronal death (n >10).

Condition	NAD⁺ (nmols/mg)	FBP (nmols/mg)
Control	2.9 ± 0.2	4.0 ± 0.3
+ Zn²⁺ preload	2.76 ± 0.2	N.P.
30 µM Ethacrynic acid (2 hr)	1.69 ± 0.15 ^*	11.3 ± 0.5 ^*
+ 10 mM pyruvate	2.27 ± 0.13 ^#	5.1 ± 0.3 ^#
+ 10 mM nicotinamide	2.35 ± 0.13 ^#	5.3 ± 0.4 ^#
+ Zn²⁺ preload	0.29 ± 0.03 ^#	N.P.
80 µM H₂O₂ (2 hr)	0.69 ± 0.03 ^*	14.3 ± 0.6 ^*
+ 10 mM nicotinamide	2.64 ± 0.18 ^#	6.4 ± 0.8 ^#
+ 10 µM TPEN	1.82 ± 0.07 ^#	7.2 ± 0.7 ^#
+ Zn²⁺ preload	0.29 ± 0.03 ^#	N.P.
Serum deprivation (6 hr)	2.01 ± 0.12 ^*	7.9 ± 0.3 ^*
+ 10 mM pyruvate	2.45 ± 0.04 ^#	5.0 ± 0.4 ^#
+ 10 mM nicotinamide	2.66 ± 0.05 ^#	5.2 ± 0.5 ^#
+ 10 µM TPEN	2.62 ± 0.2 ^#	6.2 ± 0.2 ^#
+ Zn²⁺ preload	1.39 ± 0.07 ^#	N.P.

indicates a significant difference from control.

indicates a significant difference from toxin alone at P < 0.05 by one-way ANOVA followed by a Bonferroni test.

Table 1. NAD+ levels are reduced by SD and oxidant exposures, and are restored by pyruvate, nicotinamide, and TPEN.

Table 1. NAD⁺ levels are reduced by SD and oxidant exposures, and are restored by pyruvate, nicotinamide, and TPEN.