Figure 6. TLR2-deficient mice develop increased T_H17 responses during early CAC development.

(A–D, F–H) Cytokines concentrations in colon homogenates in WT and TLR2^−/− mice at day 14 of AOM-DSS treatment were measured by ELISA. (A) IFN-g (n = 5–8). (B) IL-10 (n = 4). (C) IL-4 (n = 4–5). (D) IL-17A (n = 6). (E) IL-17A secretion from isolated chronic lamina propria cells treated with anti-CD3 (1 µg/ml) and anti-CD28 (1 µg/ml) for 72 h (n = 4). (F) TGF-ß (n = 4–10). (G) IL-23p19 (Day 0: n = 8–10; Day 14: n = 4 = 10). (H) TNFa (n = 4–10). All tests were performed using 95% confidence intervals. Data are expressed as means ± SEM. * = p<0.05, ** = p<0.01, *** = p<0.001.