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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jul 24;1801(11):1232–1237. doi: 10.1016/j.bbalip.2010.07.006

Fig. 2.

Fig. 2

Spot plate analysis of erg27 mutant yeast strains transformed with p426GPD containing the human, mouse, or yeast 3-ketoreductase. Strains were grown on SC-Ura media containing glucose supplemented with either lanosterol, ergosta-7,22-diene-3-one (precursor to Erg27p), ergosterol or Tween 80 (control). Serial dilutions of 105-102 cells were spotted onto indicated plates. Pictures were taken after 3 days growth. Strains expressing mammalian steroid-3-ketoreductase complement very poorly the erg27 yeast strain in the absence of exogenous sterol; however they complement well in the presence of ergosta-7,22-diene-3-one.