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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Jul 24;1801(11):1232–1237. doi: 10.1016/j.bbalip.2010.07.006

Table 2.

TLC separation of nonsaponifiable lipid fraction of NS0 cells incubated with [14C]acetate.

Culture
conditions		 % Total radioactivity incorporated
Squalene Oxido
squalene		 4-mono
methyl-
sterone		 Dioxidosqualene/
5α-cholestan-3-one* 		4,4-
dimethyl-
sterols		 4,4-
desmethyl-
sterols
a 4.95 3.64 51.19 2.62 23.90 13.70
b 1.11 2.21 57.43 2.73 29.15 7.37
c 5.63 4.51 58.57 3.17 15.44 12.68
d 4.64 2.29 61.18 4.48 23.65 3.76
*

Dioxidosqualene and 5α-cholestan-3-one were indistinguishable on TLC.

a

NS0 cells were pre-cultured for 2 h in the Dulbecco-modified Eagle medium (DMEM) containing 10% (v/v) lipid-depleted serum.

b

NS0 cells were pre-cultured for 24 h in the Dulbecco-modified Eagle medium (DMEM) containing 10% (v/v) lipid-depleted serum.

c

NS0 cells were pre-cultured for 2 h in the CD Hybridoma medium.

d

NS0 cells were pre-cultured for 24 h in the CD Hybridoma medium.

Results are the means of two separated experiments with duplicate incubations, each. The maximum deviations from the mean were less than 10%.