Table 3.
Incubation of homogenates from yeast strains with [14C]3-ketosteroid 1.
| Strain | % Radioactivity in nonsaponifiable lipids |
||
|---|---|---|---|
| 3-Ketosteroid 1 | 3-Hydroxysteroid 1 | Ergosterol | |
| SCY876 (wt) | 42.14 | 30.62 | 27.24 |
| BTY6-5-3 (erg27) | 92.02 | 7.98 | ND |
| STY37 (erg27/p yERG27) | 31.89 | 50.73 | 17.38 |
| STY35 (erg27/p hHSD17B7) | 39.17 | 41.57 | 19.26 |
| STY36 (erg27/p mHSD17B7) | 48.05 | 49.27 | 2.68 |
Ketosteroid 1 is a mixuture of 4-methylzymosterone, 4-methylfecosterone and 4-methylepisterone.
Results are the means of two separated experiments with duplicate incubations, each. The maximum deviations from the mean were less than 10%; ND, not detectable.