Table 4.
TLC separation of nonsaponifiable lipid fraction of S. cerevisiae cells incubated with [14C]acetate.
| Strain | % Total radioactivity incorporated |
|||||
|---|---|---|---|---|---|---|
| Squalene | Oxido squalene |
3-Keto steroid 1 |
Dioxido squalene |
Lanosterol | Ergosterol | |
| SCY876 (wt) | 14.09 | 4.58 | 3.60 | 1.63 | 8.52 | 67.58 |
| BTY6-5-3 (erg27) | 5.38 | 14.43 | 4.04 | 75.25 | 0.41 | 0.49 |
| STY37 (erg27/p yERG27) | 15.14 | 3.72 | 6.07 | 4.28 | 5.26 | 65.53 |
| STY35 (erg27/p hHSD17B7) | 2.78 | 28.93 | 3.39 | 51.94 | 3.18 | 9.78 |
| STY36 (erg27/p mHSD17B7) | 2.04 | 36.56 | 1.76 | 56.69 | 1.13 | 1.82 |
Ketosteroid 1 is a mixture of 4-methylzymosterone, 4-methylfecosterone and 4-methylepisterone.
Results are the means of two separated experiments with duplicate incubations, each. The maximum deviations from the mean were less than 10%.