Fig 3.
OCLN deletant that abrogates HCV entry can locate to cell surface. Lenti-X 293T cells expressing indicated Flag-tagged OCLN deletants were surface biotinylated or mock treated and assessed as described in Materials and Methods. All deletants were able to reach cell surface. Lysate from mock treated Flag-OCLN expressing cells was included as negative control to demonstrate the specific pull-down of biotinylated proteins.