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. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: Virology. 2010 Sep 6;407(1):160–170. doi: 10.1016/j.virol.2010.08.009

Fig. 4.

Fig. 4

OCLN EL2 is important for its association with HCV E2 in co-immunoprecipitation study. (A) 293T cells were co-transfected with indicated combinations of DNA constructs. Cell lysates were immunoprecipitated with anti-Flag M2 affinity resin at 48 hr p.i. Input cell lysates and immunoprecipitates were blotted with antibodies against HCV E1, E2, OCLN, CLDN1 as well as β-actin. Flag-OCLN co-precipitated with HCV E2 but not with E1 alone. (B) Lysates of 293T cells transfected with indicated OCLN deletant together with an E2 expressing plasmid (CMV-E1E2, H77) were immunoprecipitated with anti-Flag affinity resin. Inputs as well as immunoprecipitates were immunoblotted with indicated antibodies. The positions of Flag-OCLN/ΔN and ΔC were marked by a star sign.