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. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: Virology. 2010 Aug 25;407(1):110–119. doi: 10.1016/j.virol.2010.07.026

Fig. 5. Reducing the density of the extracellular environment affects HCV infection initiation kinetics.

Fig. 5

Huh7 cells were plated in 96-well plates, infected with HCVccwt at a MOI of 0.1 FFU/cell in cDMEM containing 1% (blue diamonds), 10% (red squares) or 20% (green circles) FBS. At indicated time points p.i., inoculum was removed from triplicate wells, intracellular RNA was collected and HCV RNA and cellular GAPDH RNA were quantified by RT-qPCR. HCV RNA is expressed as HCV copies/µg total cellular RNA. Results are graphed as mean ± stdev (n=3). * p-value = 0.05.