Huh7 cells were plated in 96-well plates and infected with equal titer amounts of (A) JFHpp, (B) H77pp or (C) VSVGpp in cDMEM containing 1% (filled diamonds), 10% (open squares) or 20% (shaded circles) FBS. At indicated time points p.i., inoculum was removed from triplicate wells, cells were washed twice with 1X PBS and overlaid with 10% cDMEM. Seventy-two hours p.i., wells were lysed and infection quantified by relative light units (RLU) ± stdev (n=3).