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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Arthritis Rheum. 2010 Sep;62(9):2680–2687. doi: 10.1002/art.27577

Figure 3.

Figure 3

The SZP concentration and aspirate volume of synovial fluid were elevated in OA samples (n = 21). Some OA samples showed higher cytokine expressions in synovial fluid. A, The total SZP content in OA samples (quantified relative to bovine standards) was elevated compared to normal samples (see text). The concentration of SZP may be indicative of the harvest procedure and could have been underestimated in OA samples with blood (OA-b) compared to those in which blood was not detected (OA-nb). B, Western blot of synovial fluids with the monoclonal antibody S6.79 recognized the large (~345 kDa) PRG4 gene product in both OA and normal patients. The same amount of SZP was loaded in each lane (based on SZP ELISA results), with OA patients having higher SZP content (and requiring less relative loads per lane) compared to normal patients. C, Some OA samples demonstrated elevated expressions of TNF-α and IL-1β cytokines in synovial fluid.